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gradient strip test  (Liofilchem)

 
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    Liofilchem gradient strip test
    Gradient Strip Test, supplied by Liofilchem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gradient+strips/concentration+e+gradient+method+strip+test/pm42119223-77-17-20
    Average 86 stars, based on 1 article reviews
    gradient strip test - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Rapid detection of susceptibility to the ceftazidime-avibactam/aztreonam combination and cefiderocol in MBL-producing Enterobacterales by MALDI-TOF MS and turbidimetry.
    Article Snippet: Introduction: Metallo-β-lactamase (MBL)-producing Enterobacterales pose a critical therapeutic challenge due to limited treatment options and high associated mortality.. Aztreonam (ATM) combined with avibactam (AVI) and cefiderocol (FDC) are the recommended treatment strategies; however, standard methods for assessing aztreonam/ceftazidime-avibactam (ATM-CZA) synergy and FDC susceptibility are time-consuming and not widely implemented.. In this context, the objective of this study was to evaluate whether matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and turbidimetry could serve as rapid, reliable tools for assessing susceptibility to ATM-CZA and FDC in MBL-producing Enterobacterales.

    Article Title: Healthcare-related transmission of mobile genetic elements co-carrying bla NDM and 16S rRNA methyltransferase genes in multiple Enterobacterales .
    Article Snippet: .. The minimum inhibitory concentration (MIC) of meropenem, gentamicin and amikacin was determined using gradient strips (Liofilchem). .. MIC was controlled using Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC® 27853.

    Isolation:

    Article Title: Rapid detection of susceptibility to the ceftazidime-avibactam/aztreonam combination and cefiderocol in MBL-producing Enterobacterales by MALDI-TOF MS and turbidimetry.
    Article Snippet: Introduction: Metallo-β-lactamase (MBL)-producing Enterobacterales pose a critical therapeutic challenge due to limited treatment options and high associated mortality.. Aztreonam (ATM) combined with avibactam (AVI) and cefiderocol (FDC) are the recommended treatment strategies; however, standard methods for assessing aztreonam/ceftazidime-avibactam (ATM-CZA) synergy and FDC susceptibility are time-consuming and not widely implemented.. In this context, the objective of this study was to evaluate whether matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and turbidimetry could serve as rapid, reliable tools for assessing susceptibility to ATM-CZA and FDC in MBL-producing Enterobacterales.

    other:

    Article Title: Emerging Clostridioides difficile strains belonging to PCR ribotype 955 in Serbia are distinct from metronidazole-resistant RT955 outbreak isolates from the UK
    Article Snippet: In Serbia, susceptibilities to five antibiotics were determined on Brucella Agar plates supplemented with haemin and vitamin K (Biomedics, Madrid, Spain) by E-test gradient strips (LIOFILCHEM ® srl, Italy), for vancomycin, tigecycline, fusidic acid, rifampicin, and moxifloxacin.



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    <t>CZA-heteroresistant</t> strains and stability of their resistant subpopulations among the 25 clinical carbapenem-resistant Klebsiella pneumoniae isolates. ( A ) <t>Satellite</t> <t>colonies</t> in the CZA E-test strip for the CRKP11, CRKP19, and CRKP26 strains. ( B ) Population analysis profiles of CRKP11, CRKP19, and CRKP26 and the control strain K. pneumoniae ATCC13883. ( C ) Time-kill kinetics assay for CRKP11, CRKP19, and CRKP26, and the control strain K. pneumoniae ATCC13883. ( D ) MIC values for the heteroresistant strains CRKP11, CRKP19, and CRKP26, and their resistant subpopulations (RS). Red lines indicate average values of three WT strain replicates. Green lines indicate average values of resistant subpopulation replicates. ( E ) MIC values of CZA for the passaged resistant subpopulations were determined at passages 0, 10, and 30 days. Shading indicates SEM values.
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    <t>CZA-heteroresistant</t> strains and stability of their resistant subpopulations among the 25 clinical carbapenem-resistant Klebsiella pneumoniae isolates. ( A ) <t>Satellite</t> <t>colonies</t> in the CZA E-test strip for the CRKP11, CRKP19, and CRKP26 strains. ( B ) Population analysis profiles of CRKP11, CRKP19, and CRKP26 and the control strain K. pneumoniae ATCC13883. ( C ) Time-kill kinetics assay for CRKP11, CRKP19, and CRKP26, and the control strain K. pneumoniae ATCC13883. ( D ) MIC values for the heteroresistant strains CRKP11, CRKP19, and CRKP26, and their resistant subpopulations (RS). Red lines indicate average values of three WT strain replicates. Green lines indicate average values of resistant subpopulation replicates. ( E ) MIC values of CZA for the passaged resistant subpopulations were determined at passages 0, 10, and 30 days. Shading indicates SEM values.
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    Image Search Results


    Distribution of MIC values from ETEST AZA and BMD on clinical and challenge isolates. ( A ) FDA breakpoints ( N = 602) and ( B ) EUCAST breakpoints ( N = 650), with their respective interpretation noted on the top and left of the plot. Gray rectangles represent MICs in essential agreement, defined as MICs within ±1 doubling dilution. Abbreviations: MIC, minimal inhibitory concentration; AZA, aztreonam-avibactam; BMD, broth microdilution; S, susceptible; I, intermediate; R, resistant.

    Journal: Journal of Clinical Microbiology

    Article Title: A multicenter performance evaluation of aztreonam-avibactam gradient diffusion susceptibility testing for Enterobacterales

    doi: 10.1128/jcm.01749-25

    Figure Lengend Snippet: Distribution of MIC values from ETEST AZA and BMD on clinical and challenge isolates. ( A ) FDA breakpoints ( N = 602) and ( B ) EUCAST breakpoints ( N = 650), with their respective interpretation noted on the top and left of the plot. Gray rectangles represent MICs in essential agreement, defined as MICs within ±1 doubling dilution. Abbreviations: MIC, minimal inhibitory concentration; AZA, aztreonam-avibactam; BMD, broth microdilution; S, susceptible; I, intermediate; R, resistant.

    Article Snippet: One other gradient diffusion method has been evaluated, the Liofilchem aztreonam-avibactam gradient strip ( ).

    Techniques: Concentration Assay

    CZA-heteroresistant strains and stability of their resistant subpopulations among the 25 clinical carbapenem-resistant Klebsiella pneumoniae isolates. ( A ) Satellite colonies in the CZA E-test strip for the CRKP11, CRKP19, and CRKP26 strains. ( B ) Population analysis profiles of CRKP11, CRKP19, and CRKP26 and the control strain K. pneumoniae ATCC13883. ( C ) Time-kill kinetics assay for CRKP11, CRKP19, and CRKP26, and the control strain K. pneumoniae ATCC13883. ( D ) MIC values for the heteroresistant strains CRKP11, CRKP19, and CRKP26, and their resistant subpopulations (RS). Red lines indicate average values of three WT strain replicates. Green lines indicate average values of resistant subpopulation replicates. ( E ) MIC values of CZA for the passaged resistant subpopulations were determined at passages 0, 10, and 30 days. Shading indicates SEM values.

    Journal: Microbiology Spectrum

    Article Title: Tandem amplification of the umpA allele contributes to ceftazidime-avibactam heteroresistance in clinical carbapenem-resistant Klebsiella pneumoniae isolates

    doi: 10.1128/spectrum.01722-25

    Figure Lengend Snippet: CZA-heteroresistant strains and stability of their resistant subpopulations among the 25 clinical carbapenem-resistant Klebsiella pneumoniae isolates. ( A ) Satellite colonies in the CZA E-test strip for the CRKP11, CRKP19, and CRKP26 strains. ( B ) Population analysis profiles of CRKP11, CRKP19, and CRKP26 and the control strain K. pneumoniae ATCC13883. ( C ) Time-kill kinetics assay for CRKP11, CRKP19, and CRKP26, and the control strain K. pneumoniae ATCC13883. ( D ) MIC values for the heteroresistant strains CRKP11, CRKP19, and CRKP26, and their resistant subpopulations (RS). Red lines indicate average values of three WT strain replicates. Green lines indicate average values of resistant subpopulation replicates. ( E ) MIC values of CZA for the passaged resistant subpopulations were determined at passages 0, 10, and 30 days. Shading indicates SEM values.

    Article Snippet: CZA heteroresistance in the K. pneumoniae clinical isolates was confirmed by PAP for colonies growing within the inhibitory zone in the CZA gradient strip testing (Liofilchem, Roseto degli Abruzzi, Italy).

    Techniques: Stripping Membranes, Control